The Blood Transfusion Centre, Orebro.
Platelets. 1993;4(4):197-200. doi: 10.3109/09537109309013217.
The present work investigates the effects of contamination with Pseudomonas aeruginosa on some properties of platelet concentrates, i.e. the ability to transmit light, swirling and the degree of platelet activation and cell lysis. 20 pairs of platelet concentrates were studied over a 7 day period. On day 1, one of the concentrates was inoculated with Pseudomonas aeruginosa, while the other served as an identical non-contaminated control. In the contaminated concentrates, swirling and release of platelet factor 4 and lactate dehydrogenase were analysed on day 1, day 2 and day 3; bacterial counts were performed on day 2 and day 3, and on day 7 all these variables were determined in both concentrates. After the 7 day storage period, bacterial growth was found in 13/16 of the contaminated platelet concentrates; all these preparations displayed transmission changes and at least one deviating biochemical parameter. On day 3 all contaminated concentrates had unimpaired swirling, 15/20 had bacterial growth and 10/20 light transmission changes. The study demonstrates that light transmission monitoring on day 3 offers significant advantage (p < 0.05) compared to the visual estimation of swirling. The results also show that the optical method often fails to identify contaminated concentrates during early storage.
本研究调查了铜绿假单胞菌污染对血小板浓缩物某些性质的影响，即透光性、漩涡形成能力、血小板活化和细胞溶解程度。对 20 对血小板浓缩物进行了为期 7 天的研究。第 1 天，将其中一个浓缩物接种铜绿假单胞菌，另一个作为相同的无污染对照。在污染的浓缩物中，在第 1、2 和 3 天分析漩涡形成能力和血小板因子 4 和乳酸脱氢酶的释放情况；在第 2 和第 3 天进行细菌计数，并在第 7 天确定这两种浓缩物中的所有这些变量。经过 7 天的储存期，在 13/16 份污染的血小板浓缩物中发现了细菌生长；所有这些制剂都显示出透光性变化，并且至少有一个生化参数偏离。在第 3 天，所有污染的浓缩物都有未受损的漩涡形成能力，15/20 有细菌生长，10/20 有透光性变化。该研究表明，与漩涡的视觉评估相比，第 3 天的透光性监测具有显著优势（p < 0.05）。结果还表明，在早期储存期间，光学方法经常无法识别污染的浓缩物。
