Jaypee University of Information Technology, Waknaghat, India.
Int Braz J Urol. 2010 Sep-Oct;36(5):621-8. doi: 10.1590/s1677-55382010000500013.
Investigate the activity of high and low molecular weight biomolecules present in the matrix of human calcium oxalate (CaOx) stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization of the preformed mineral phase.
Surgically removed renal stones were analyzed by Fourier Transform Infra Red (FTIR) spectroscopy and only CaOx stones were extracted with 0.05M EGTA, 1 mM PMSF and 1% Β-mercaptoethanol. Renal CaOx stone extract was separated into > 10 kDa and < 10 kDa fractions by dialysis. Activity of both the fractions along with whole extract was studied on the three mineral phases of CaP assay system.
It was interesting to observe that both high and low molecular weight biomolecules extracted from human renal matrix of calcium oxalate (CaOx) stones exhibited different roles in the three mineral phases of CaP. Whole extract exhibited inhibitory activity in all the three assay systems; however, mixed (stimulatory and inhibitory) activity was exhibited by the > 10 kDa and < 10 kDa fractions. SDS-PAGE analysis showed bands of 66 kDa, 80 kDa, 42 kDa in whole EGTA extract lane and > 10 kDa fraction lane.
Both high and low molecular weight biomolecules extracted from human renal matrix of calcium oxalate (CaOx) stones have a significant influence on calcium and phosphate (CaP) crystallization.
研究人草酸钙（CaOx）结石基质中高分子量和低分子量生物分子的活性，不仅对钙和磷（CaP）的初始矿物相形成有影响，而且对预先形成的矿物相的生长和脱矿也有影响。
通过傅里叶变换红外（FTIR）光谱分析手术切除的肾结石，仅用 0.05M EGTA、1mM PMSF 和 1%Β-巯基乙醇从结石中提取草酸钙（CaOx）。将肾草酸钙结石提取物通过透析分离成>10 kDa 和<10 kDa 两个部分。研究了这两个部分以及整个提取物在 CaP 测定系统的三种矿物相中对 CaP 的活性。
有趣的是，从人草酸钙（CaOx）结石的肾基质中提取的高分子量和低分子量生物分子在 CaP 的三种矿物相中表现出不同的作用。整个提取物在所有三个测定系统中都表现出抑制活性，而>10 kDa 和<10 kDa 部分则表现出混合（刺激和抑制）活性。SDS-PAGE 分析显示在整个 EGTA 提取物泳道和>10 kDa 部分泳道中存在 66 kDa、80 kDa、42 kDa 的条带。
从人草酸钙（CaOx）结石的肾基质中提取的高分子量和低分子量生物分子对钙和磷（CaP）结晶有显著影响。
Zotero 插件