Universidad de Oviedo, Facultad de Psicología, Oviedo, Spain.
Psicothema. 2010 Nov;22(4):893-7.
We evaluated change in cytochrome oxidase (COx) activity of the hippocampus and related structures of the limbic system following spatial working memory learning in rats after treatment with tacrine (8.0mg/kg). Control groups treated with saline and tacrine and an untreated group were added. Acetylcholinesterase optical density levels were also measured. The tacrine and saline groups showed similar behavioral results, but a decrease in COx activity was found in the tacrine group in the prefrontal cortex, nucleus accumbens, anterior thalamus, hippocampus and nucleus basalis of Meynert. Similarly, acetylcholinesterase levels of the tacrine group were lower in most of the regions. Learning-related increase in COx activity was found in the prefrontal cortex and dentate gyrus in the saline group. The tacrine group presented the same increase in the anterodorsal thalamus, dentate gyrus, CA3 and mammillary nuclei. These results suggest that inhibition of the acetylcholinesterase produces a different pattern of learning-related neuronal activity in the limbic system of the rat.
我们评估了在给予他克林（8.0mg/kg）后，大鼠在进行空间工作记忆学习时，海马体和边缘系统相关结构的细胞色素氧化酶（COx）活性的变化。还添加了用生理盐水和他克林处理的对照组以及未经处理的组。还测量了乙酰胆碱酯酶的光密度水平。他克林组和生理盐水组表现出相似的行为结果，但在他克林组的前额叶皮层、伏隔核、前丘脑、海马体和梅内尔特基底核中发现 COx 活性降低。同样，他克林组的大多数区域中的乙酰胆碱酯酶水平较低。在生理盐水组中，COx 活性在额前皮质和齿状回中与学习相关增加。他克林组在前背侧丘脑、齿状回、CA3 和乳头体中也表现出相同的增加。这些结果表明，乙酰胆碱酯酶的抑制在大鼠的边缘系统中产生了不同的与学习相关的神经元活动模式。
