自发扭转对 DNA 迷你环的影响。
Effect of spontaneous twist on DNA minicircles.
机构信息
Department of Physics, Bar-Ilan University, Ramat-Gan, Israel.
出版信息
Biophys J. 2010 Nov 3;99(9):2987-94. doi: 10.1016/j.bpj.2010.08.021.
Abstract
Monte Carlo simulations are used to study the effect of spontaneous (intrinsic) twist on the conformation of topologically equilibrated minicircles of dsDNA. The twist, writhe, and radius of gyration distributions and their moments are calculated for different spontaneous twist angles and DNA lengths. The average writhe and twist deviate in an oscillatory fashion (with the period of the double helix) from their spontaneous values, as one spans the range between two neighboring integer values of intrinsic twist. Such deviations vanish in the limit of long DNA plasmids.
摘要
我们使用蒙特卡罗模拟来研究自发（内在）扭曲对拓扑平衡的 dsDNA 迷你环构象的影响。我们计算了不同自发扭曲角和 DNA 长度下的扭曲、纽结和旋转半径分布及其矩。当跨越内在扭曲的两个相邻整数值的范围时，平均纽结和扭曲会以双螺旋的周期为特征呈现出波动的偏离（相对于它们的自发值）。这种偏差在长 DNA 质粒的极限情况下消失。
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Biophys J. 2010 Nov 3;99(9):2987-94. doi: 10.1016/j.bpj.2010.08.021.
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