Department of Biochemistry, Faculty of Biology, Havana University, Ave. 25, No. 455, Vedado, Ciudad de La Habana, Cuba, CP 10400.
Comp Biochem Physiol B Biochem Mol Biol. 2011 Feb;158(2):136-41. doi: 10.1016/j.cbpb.2010.10.009. Epub 2010 Oct 31.
The variation of activity of some digestive enzymes was studied in three age groups of two freshwater endemic fishes from Cuba: Limia vittata and Gambusia punctata. Trypsin, chymotrypsin and amylase activities showed a different pattern between both species. Trypsin and chymotrypsin activity increased with the age of fishes, while amylase activity decreased. The highest activity of trypsin and chymotrypsin was registered in G. punctata while the highest amylase activity was detected in L. vittata. Zymograms revealed proteases with molecular masses similar to trypsin and chymotrypsin reported for other fish species. Amylase electrophoresis showed the presence of this enzyme; in L. vittata amylase zymograms showed two bands with molecular masses of 175 and 100 kDa and in G. punctata four bands of 175, 100, 46 and 30 kDa respectively were found. The activity of the digestive enzymes can be used as an effective indicator of the feeding habits and the development of the digestive tracts in L. vittata and G. punctata.
Limia vittata 和 Gambusia punctata 的三个年龄组的一些消化酶的活性变化。胰蛋白酶、糜蛋白酶和淀粉酶活性在两种鱼类之间呈现出不同的模式。胰蛋白酶和糜蛋白酶活性随鱼类年龄的增长而增加，而淀粉酶活性则降低。G. punctata 中的胰蛋白酶和糜蛋白酶活性最高，而 L. vittata 中的淀粉酶活性最高。同工酶图谱显示，蛋白酶的分子量与其他鱼类报道的胰蛋白酶和糜蛋白酶相似。淀粉酶电泳显示存在这种酶；在 L. vittata 中，淀粉酶同工酶图谱显示两条分子量为 175 和 100 kDa 的带，而在 G. punctata 中则发现 4 条分子量分别为 175、100、46 和 30 kDa 的带。消化酶的活性可以作为 L. vittata 和 G. punctata 摄食习性和消化道发育的有效指标。
