The use of tears for diagnosis of GM1 gangliosidosis.
作者信息
Tsuboyama A, Miki F, Yoshida M, Ogura Y, Miyatake T
出版信息
Clin Chim Acta. 1977 Oct 15;80(2):237-42. doi: 10.1016/0009-8981(77)90030-4.
PMID:21045
Abstract
The properties of beta-galactosidase of tears were investigated and the standard assay system was accomplished. The pH optimum was 4.2. The enzyme had a KM of 8.3 X 10(-4) M. The activity was stimulated by chloride ions and slightly stabilized by bovine serum albumin. The activities of normal individuals were 205 +/- 80 (S.D.) nmol/h/ml. The activity in the tears of the patient with GM1 gangliosidosis decreased to about 20% of normal control and this disease could be diagnosed by the assay of beta-galactosidase in tears.
摘要
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