红三叶草坏死花叶病毒的结晶及初步X射线衍射分析
Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus.
作者信息
Martin Stanton L, Guenther Richard H, Sit Tim L, Swartz Paul D, Meilleur Flora, Lommel Steven A, Rose Robert B
机构信息
Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Nov 1;66(Pt 11):1458-62. doi: 10.1107/S1744309110032483. Epub 2010 Oct 28.
Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X-ray analysis using the hanging-drop vapor-diffusion method. Self-rotation functions and systematic absences identified the space group as I23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. The data were processed to 6 Å in the analysis presented here.
红三叶草坏死花叶病毒（RCNMV）是一种属于番茄病毒科的植物病毒，具有T = 3二十面体衣壳。RCNMV病毒粒子经过纯化，并使用悬滴气相扩散法进行结晶以用于X射线分析。自旋转函数和系统消光确定空间群为I23，晶胞中有两个病毒粒子。这些晶体的衍射分辨率优于4 Å，但对辐射非常敏感，导致高分辨率反射迅速衰减。在此处呈现的分析中，数据处理至6 Å。
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