Brito José A, Borges Nuno, Santos Helena, Archer Margarida
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, EAN, 2780-157 Oeiras, Portugal.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Nov 1;66(Pt 11):1463-5. doi: 10.1107/S1744309110032677. Epub 2010 Oct 28.
Archaeoglobus fulgidus, a hyperthermophilic archaeon, accumulates di-myo-inositol phosphate (DIP) in response to heat stress. Recently, the pathway for biosynthesis of DIP has been elucidated in this organism and involves a bifunctional enzyme that contains two domains: CTP:inositol-1-phosphate cytidylyltransferase (IPCT) as a soluble domain and di-myo-inositol-1,3'-phosphate-1-phosphate synthase (DIPPS) as a membrane domain. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis of the IPCT domain from A. fulgidus in the apo form are reported. The crystals diffracted to 2.4 Å resolution using a synchrotron source and belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 154.7, b = 83.9, c = 127.7 Å.
嗜热栖热菌（Archaeoglobus fulgidus）是一种嗜热古菌，在热应激条件下会积累二 - 肌醇磷酸（DIP）。最近，该生物体中DIP的生物合成途径已被阐明，涉及一种双功能酶，它包含两个结构域：作为可溶性结构域的CTP：肌醇 - 1 - 磷酸胞苷转移酶（IPCT）和作为膜结构域的二 - 肌醇 - 1,3'-磷酸 - 1 - 磷酸合酶（DIPPS）。本文报道了嗜热栖热菌中IPCT结构域无配体形式的表达、纯化、结晶及初步X射线衍射分析。使用同步辐射源，晶体衍射分辨率达到2.4 Å，属于正交晶系空间群P2(1)2(1)2，晶胞参数a = 154.7，b = 83.9，c = 127.7 Å。
