Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg, Illkirch, France.
RNA Biol. 2010 Nov-Dec;7(6):687-99. doi: 10.4161/rna.7.6.13616. Epub 2010 Nov 1.
The nucleocapsid protein NCp7 of HIV-1 possesses nucleic acid chaperone properties that are thought to be crucial throughout the viral life cycle. These properties promote the rearrangement of nucleic acids into their most thermodynamically stable conformations. These NCp7 properties involve two components, namely nucleic acid destabilization and activation of the annealing of complementary sequences. Biophysical techniques have been found extremely powerful to decipher the molecular mechanisms underlying these two components. We propose here an overview of the recent reports that examine the nucleic acid chaperone properties of NCp7 by these techniques.
HIV-1 的核衣壳蛋白 NCp7 具有核酸伴侣性质，这些性质被认为在整个病毒生命周期中至关重要。这些性质促进了核酸进入其最稳定的构象。这些 NCp7 性质涉及两个组成部分，即核酸的去稳定化和互补序列退火的激活。生物物理技术已被发现非常强大，可以解析这两个组成部分背后的分子机制。我们在这里提出了一个综述，介绍了这些技术研究 NCp7 核酸伴侣性质的最新报告。
