Department of Ophthalmology, Toranomon Hospital, Tokyo, Japan.
Cornea. 2011 May;30(5):524-7. doi: 10.1097/ICO.0b013e3181f23932.
To evaluate whether Immfast Check J1, a new commercial immunochromatographic test for the measurement of specific immunoglobulin (Ig) E, could be used as a screening test for the diagnosis of allergic conjunctivitis.
A prospective, nonrandomized, cross-sectional study was conducted in 64 moderate to severe cases of allergic conjunctivitis (allergic group) and 44 age- and sex-matched healthy control subjects (control group). Specific IgE levels for cedar pollen, cat epithelium, and Dermatophagoides pteronyssinus were measured in tear fluid with the Immfast Check J1.
Specific IgE levels in tear fluid could be assayed within 20 minutes in all subjects. The positive rate of specific IgE was significantly higher in the allergic group than in the control group (cedar pollen, 96.9% vs. 2.3%, P < 0.00001; cat epithelium, 23.4% vs. 0.0%, P = 0.00018; and D. pteronyssinus, 53.1% vs. 2.3%, P < 0.00001). Specific IgE scores were also significantly higher in the allergic group than in the control group (cedar pollen, P < 0.00001; cat epithelium, P = 0.03907; and D. pteronyssinus, P < 0.00001).
These results suggest that Immfast Check J1 is a rapid and easy test for detection of allergen-specific IgE in tear fluid samples from patients with allergic conjunctivitis. The test is reliable and easy to perform on an outpatient basis.
评估 Immfast Check J1（一种用于测量特异性免疫球蛋白 (Ig) E 的新商业免疫层析检测法）是否可用作过敏性结膜炎诊断的筛选检测法。
前瞻性、非随机、横断面研究共纳入 64 例中重度过敏性结膜炎患者（过敏性组）和 44 名年龄和性别匹配的健康对照者（对照组）。采用 Immfast Check J1 检测泪液中的 cedar 花粉、猫上皮细胞和屋尘螨特异性 IgE。
所有受试者均能在 20 分钟内完成泪液中特异性 IgE 的检测。过敏性组的特异性 IgE 阳性率显著高于对照组（cedar 花粉，96.9% vs. 2.3%，P<0.00001；猫上皮细胞，23.4% vs. 0.0%，P=0.00018；屋尘螨，53.1% vs. 2.3%，P<0.00001）。过敏性组的特异性 IgE 评分也显著高于对照组（cedar 花粉，P<0.00001；猫上皮细胞，P=0.03907；屋尘螨，P<0.00001）。
这些结果表明，Immfast Check J1 是一种快速、简便的检测方法，可用于检测过敏性结膜炎患者的泪液样本中的过敏原特异性 IgE。该检测法可靠，易于在门诊进行。
