Chen Huiqing, Li Yijia, Chen Keping, Yao Qin, Li Guohui, Wang Lin
Institute of Life Sciences, Jiangsu University, Zhenjiang, P R China.
Acta Biochim Pol. 2010;57(4):505-11. Epub 2010 Nov 2.
Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
众所周知，热量限制（CR）能够延长从酵母到哺乳动物的寿命。为了从蛋白质水平深入了解热量限制对家蚕生长发育的影响，我们采用比较蛋白质组学方法，研究了经历热量限制的家蚕幼虫血淋巴和脂肪体的蛋白质组差异。通过基质辅助激光解吸电离飞行时间串联质谱（MALDI TOF/TOF MS）鉴定出39种差异表达蛋白。其中，19种来自血淋巴，20种来自脂肪体。热量限制组的血淋巴中有2种下调蛋白和17种上调蛋白，而脂肪体中有15种下调蛋白和5种上调蛋白。这些蛋白质属于在免疫系统、信号转导和细胞凋亡、生长发育调节以及能量代谢中发挥作用的蛋白质。我们的结果表明，热量限制可以改变与上述四个方面相关的蛋白质表达，这意味着这些蛋白质可能通过热量限制调节家蚕的寿命。
