Department of Biophotonics Research, Gloucestershire Hospitals NHS Foundation Trust, Gloucester Royal Hospital, Leadon House, Gloucester, Glos, UK GL1 3NN.
Analyst. 2010 Dec;135(12):3042-7. doi: 10.1039/c0an00527d. Epub 2010 Nov 2.
Sentinel Lymph Node Biopsy has become the standard surgical procedure for the sampling of axillary lymph nodes in breast cancer. Intra operative node assessment is currently not offered to the majority of patients but would allow definitive axillary surgery to take place immediately. This would confer benefits both to the patient and to the healthcare system. Our experimental study aims to demonstrate that a Raman spectroscopy probe device could overcome many of the disadvantages of current intra-operative analysis techniques. 38 axillary lymph nodes, 25 negative and 13 positive from 20 patients undergoing breast surgery for invasive breast cancer were assessed using a commercially available Raman spectroscopy probe. Spectra were assessed using principal component fed linear discriminant analysis trained by the histopathology results. Leave one node out cross validation achieved a sensitivity of up to 92% and a specificity of up to 100% in differentiating between normal and metastatic lymph nodes.
前哨淋巴结活检已成为乳腺癌腋窝淋巴结取样的标准手术程序。目前，大多数患者都无法进行术中淋巴结评估，但这将允许立即进行确定性腋窝手术。这将使患者和医疗保健系统都受益。我们的实验研究旨在证明拉曼光谱探头设备可以克服当前术中分析技术的许多缺点。我们使用商业上可用的拉曼光谱探头评估了 20 名接受浸润性乳腺癌乳房手术的患者的 38 个腋窝淋巴结，其中 25 个为阴性，13 个为阳性。使用主成分线性判别分析对基于组织病理学结果进行训练的光谱进行评估。采用留一节点交叉验证的方法，在区分正常和转移性淋巴结方面，灵敏度高达 92%，特异性高达 100%。
