Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Bioprocess Biosyst Eng. 2011 Mar;34(3):347-55. doi: 10.1007/s00449-010-0477-8. Epub 2010 Nov 3.
Solid-state fermentation obtained from different and low-cost carbon sources was evaluated to endocellulases and endoxylanases production by Aspergillus japonicus C03. Regarding the enzymatic production the highest levels were observed at 30 °C, using soy bran added to crushed corncob or wheat bran added to sugarcane bagasse, humidified with salt solutions, and incubated for 3 days (xylanase) or 6 days (cellulase) with 70% relative humidity. Peptone improved the xylanase and cellulase activities in 12 and 29%, respectively. The optimum temperature corresponded to 60 °C and 50-55 °C for xylanase and cellulase, respectively, both having 4.0 as optimum pH. Xylanase was fully stable up to 40 °C, which is close to the rumen temperature. The enzymes were stable in pH 4.0-7.0. Cu++ and Mn++ increased xylanase and cellulase activities by 10 and 64%, respectively. A. japonicus C03 xylanase was greatly stable in goat rumen fluid for 4 h during in vivo and in vitro experiments.
采用不同的低成本碳源固态发酵生产内切葡聚糖酶和内切木聚糖酶的方法。在酶生产方面，使用添加到粉碎的玉米芯中的大豆糠或添加到甘蔗渣中的麦麸，用盐溶液加湿，在 30°C 下培养 3 天（木聚糖酶）或 6 天（纤维素酶），相对湿度为 70%时，酶活最高。蛋白胨分别提高了木聚糖酶和纤维素酶的活性 12%和 29%。最适温度分别为 60°C 和 50-55°C，最适 pH 分别为 4.0 和 50-55°C。木聚糖酶在 40°C 以下完全稳定，接近瘤胃温度。该酶在 pH4.0-7.0 下稳定。Cu++和 Mn++分别提高木聚糖酶和纤维素酶的活性 10%和 64%。在体内和体外实验中，A. japonicus C03 木聚糖酶在山羊瘤胃液中 4 小时内非常稳定。
