Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, São José dos Campos, SP, Brazil.
J Fluoresc. 2011 Mar;21(2):733-8. doi: 10.1007/s10895-010-0763-5. Epub 2010 Nov 3.
A simple, rapid and effective analytical method based on fluorescence spectroscopy for the determination of coumarin in pharmaceutical formulations without pre-treatment or pre-concentration step was development. Coumarin had maximum excitation and emission at 310 nm and 390 nm, respectively. Optimum conditions for the detection of coumarin were investigated. Under optimized conditions, we observed a linear behavior for the sign of coumarin in the concentration range of 2.5 × 10(-6) to 1.0 × 10(-4) mol L(-1), with linearity of 0.998 and sensitivity of 2.9 × 10(10) u.a/mol L(-1). The proposed method was validated in terms of accuracy, precision and specificity of coumarin using the standard addition and external calibration. It was noted that the results support (P<0.05), indicating that the matrices were not an interference in the determination of coumarin by fluorescence spectroscopy. The results were favorable compared with those obtained by reference chromatographic methods.
建立了一种无需预处理或预浓缩步骤即可直接测定药物制剂中香豆素的简单、快速、有效的荧光光谱分析方法。香豆素的最大激发和发射波长分别为 310nm 和 390nm。考察了检测香豆素的最佳条件。在优化条件下，我们观察到香豆素在 2.5×10(-6)至 1.0×10(-4)mol L(-1)浓度范围内的信号呈线性关系，线性度为 0.998，灵敏度为 2.9×10(10)u.a/mol L(-1)。采用标准加入和外标校准法对香豆素的准确性、精密度和特异性进行了验证。结果表明（P<0.05），表明荧光光谱法测定香豆素时，基质没有干扰。与参考色谱法得到的结果相比，该方法结果良好。
