He Tao, Deng Li, Lin Yuan, Li Bo, Yang Xiaofan, Wang Fang, Chun Ze
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2124-8.
To provide theoretical basis for breeding good variety of Dendrobium denneanum, agronomic and qualitative characters of 4 different lines and relationships among them were studied. The stem length, stem diameter, leaf length, leaf width, length/ width ratio and leaf area were measured. The single fresh and dry stem was weighed and drying rate was calculated. The contents of polysaccharides and total alkaloids were determined by sulfuric acid-phenol colorimetry and acid-dye colorimetry, respectively. The correlations between characters were analyzed. The results showed that differences in major agronomic characters between four lines were significant. The plant types of dq-1 and dq-2 were higher, dq-3 was medium and dq-4 was lower. The fresh weigh of stem and content of polysaccharides were the highest in dq-2, 7.81 g and 14.33%. While the highest content of total alkaloids and was 0. 486% in dq-3. There were significant correlations between agronomic characters, but these characters had low or non correlations with qualitative characters such as polysaccharides and total alkaloids. It was shown that the content of polysaccharides and total alkaloids were significantly different among 4 lines of D. denneanum, which could be selected for different uses.
为给滇金石斛优良品种选育提供理论依据,对4个不同株系的农艺性状、品质性状及其相互关系进行了研究。测定了茎长、茎粗、叶长、叶宽、长宽比和叶面积。称量单茎鲜重和干重并计算干鲜比。分别采用硫酸-苯酚比色法和酸性染料比色法测定多糖和总生物碱含量。分析各性状间的相关性。结果表明,4个株系主要农艺性状差异显著。dq-1和dq-2株型较高,dq-3中等,dq-4较低。dq-2茎鲜重和多糖含量最高,分别为7.81 g和14.33%。而dq-3总生物碱含量最高,为0.486%。农艺性状间存在显著相关性,但这些性状与多糖和总生物碱等品质性状相关性低或无相关性。结果表明,4个滇金石斛株系多糖和总生物碱含量差异显著,可根据不同用途进行选择。