González-Hein Gisela, González-Hein Jaime, Díaz Jarabrán Maria C
Agriculture and Veterinary Sciences, University of Chile, Avenida Santa Rosa 11.735, La Pintana, Santiago, Chile.
J Avian Med Surg. 2010 Sep;24(3):227-36. doi: 10.1647/2009-003.1.
To investigate the prevalence of Cryptococcus in droppings from captive birds in Chile, dry droppings from 113 captive birds of various species were cultured for Cryptococcus neoformans. The yeast was recovered from 17 of the 113 samples (15% [95% confidence intervals, 8.4%-21.6%]). Other yeast organisms recovered from psittacine bird droppings were Cryptococcus albidus and Cryptococcus uniguttulatus. Secreted phospholipase has been proposed as a virulence determinant in C neoformans. Phospholipase production by the egg yolk plate method, and in vitro susceptibility to fluconazole by using the disk diffusion test were performed on 17 C neoformans isolates. Two of the 17 strains (11.7%) did not produce phospholipase. Two (11.7%) were resistant to fluconazole, and 5 of 17 (29.4%) were susceptible dose-dependent. The Cryptococcus species isolated from droppings from captive birds could be potential pathogens in humans.
为调查智利圈养鸟类粪便中隐球菌的流行情况，对113只不同种类圈养鸟类的干燥粪便进行新型隐球菌培养。在113份样本中的17份（15%[95%置信区间，8.4%-21.6%]）中分离出了该酵母菌。从鹦鹉粪便中分离出的其他酵母菌有浅白隐球菌和单孢隐球菌。分泌型磷脂酶被认为是新型隐球菌的一种毒力决定因素。采用蛋黄平板法对17株新型隐球菌分离株进行磷脂酶产生情况检测，并采用纸片扩散法检测其对氟康唑的体外敏感性。17株菌株中有2株（11.7%）不产生磷脂酶。2株（11.7%）对氟康唑耐药，17株中有5株（29.4%）对氟康唑呈剂量依赖性敏感。从圈养鸟类粪便中分离出的隐球菌种类可能是人类的潜在病原体。
