El-Beshbishy Hesham A, Mohamadin Ahmed M, Nagy Ayman A, Abdel-Naim Ashraf B
Medical Laboratories Technology Dept., Facuty of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia.
Indian J Exp Biol. 2010 Mar;48(3):280-8.
Liver injury was induced in female rats using tamoxifen (TAM). Grape seeds (Vitis vinifera) extract (GSE), black seed (Nigella sativa) extract (NSE), curcumin (CUR) or silymarin (SYL) were orally administered to TAM-intoxicated rats. Liver histopathology of TAM-intoxicated:rats showed pathological changes. TAM-intoxication elicited declines in liver antioxidant enzymes levels (glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase), reduced glutathione (GSH) and GSH/GSSG ratio plus the hepatic elevations in lipid peroxides, oxidized glutathione (GSSG), tumor necrosis factor-alpha (TNF-alpha) and serum liver enzymes; alanine transaminase, aspartate transaminase, alkaline phosphatase, lactate dehydrogenase and gamma glutamyl transferase levels. Oral intake of NSE, GSE, CUR or SYL to TAM-intoxicated rats, attenuated histopathological changes and corrected all parameters mentioned above. Improvements were prominent in case of NSE (similarly SYL) > CUR > GSE. Data indicated that NSE, GSE or CUR act as free radicals scavengers and protect TAM-induced liver injury in rats.
使用他莫昔芬（TAM）诱导雌性大鼠肝损伤。将葡萄籽（葡萄）提取物（GSE）、黑种草籽提取物（NSE）、姜黄素（CUR）或水飞蓟宾（SYL）口服给予TAM中毒的大鼠。TAM中毒大鼠的肝脏组织病理学显示出病理变化。TAM中毒导致肝脏抗氧化酶水平（谷胱甘肽过氧化物酶、谷胱甘肽还原酶、超氧化物歧化酶和过氧化氢酶）、还原型谷胱甘肽（GSH）和GSH/GSSG比值下降，同时脂质过氧化物、氧化型谷胱甘肽（GSSG）、肿瘤坏死因子-α（TNF-α）和血清肝酶（丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、乳酸脱氢酶和γ-谷氨酰转移酶水平）升高。给TAM中毒的大鼠口服NSE、GSE、CUR或SYL，可减轻组织病理学变化并纠正上述所有参数。NSE（与SYL相似）>CUR>GSE时改善最为显著。数据表明，NSE、GSE或CUR可作为自由基清除剂，保护大鼠免受TAM诱导的肝损伤。
