School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, United States.
J Am Chem Soc. 2010 Nov 24;132(46):16334-6. doi: 10.1021/ja107259y. Epub 2010 Nov 3.
The majority of charge-compensating ions around nucleic acids form a diffuse counterion "cloud" that is not amenable to investigation by traditional methods that rely on rigid structural interactions. Although various techniques have been employed to characterize the ion atmosphere around nucleic acids, only anomalous small-angle X-ray scattering (ASAXS) provides information about the spatial distribution of ions. Here we present an experimentally straightforward extension of ASAXS that can be used to count the number of ions around nucleic acids.
大多数在核酸周围的电荷补偿离子形成一个弥散的抗衡离子“云”，这是不容易用传统的方法来研究，传统方法依赖于刚性的结构相互作用。虽然已经采用了各种技术来描绘核酸周围的离子环境，但只有异常小角 X 射线散射 (ASAXS) 提供了关于离子空间分布的信息。在这里，我们提出了一个实验上简单的 ASAXS 扩展，可以用来计算核酸周围的离子数量。
