Bannantine John P, Talaat Adel M
National Animal Disease Center, USDA - Agricultural Research Service, 2300 North Dayton Avenue, Ames, IA, USA.
Vet Immunol Immunopathol. 2010 Dec 15;138(4):303-11. doi: 10.1016/j.vetimm.2010.10.008. Epub 2010 Nov 2.
Microarray technology is an important tool in functional genomic research. It has enabled a deeper analysis of genomic diversity among bacteria belonging to the Mycobacterium avium complex (MAC). In addition, the expression of thousands of genes can be studied simultaneously in a single experiment. With the complete genome sequence of a bovine isolate of M. avium subspecies paratuberculosis, and the independent construction of DNA microarrays in our laboratories, transcriptomic studies for this veterinary pathogen are now possible. Furthermore, the bovine genome sequence project is completed and bovine arrays have been developed to examine host responses to infection with M. avium subsp. paratuberculosis. Collectively, genomic and transcriptomic data has yielded novel insights surrounding the genetic regulation and biology of Johne's disease.
微阵列技术是功能基因组研究中的一项重要工具。它使得对鸟分枝杆菌复合群（MAC）中的细菌基因组多样性有了更深入的分析。此外，在单个实验中可以同时研究数千个基因的表达。随着鸟分枝杆菌副结核亚种牛分离株的全基因组序列的完成，以及我们实验室独立构建DNA微阵列，现在可以对这种兽医病原体进行转录组学研究。此外，牛基因组序列项目已经完成，并且已经开发出牛微阵列来检测宿主对鸟分枝杆菌副结核亚种感染的反应。总体而言，基因组和转录组数据为约翰氏病的遗传调控和生物学提供了新的见解。
