The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Biol Pharm Bull. 2010;33(11):1822-7. doi: 10.1248/bpb.33.1822.
Small noncoding microRNAs (miRNAs) have been shown to play an important role in tumor proliferation and metastasis. However, their function and mechanism in the proliferation and metastasis of gastric cancer has not yet been elucidated. Here, we investigated the relationship between miRNA-199a and gastric cancer proliferation and metastasis. Using real-time reverse-transcriptase (RT)-polymerase chain reaction, we found that miR-199a is highly expressed in gastric cancer compared to normal gastric tissues and in metastatic, compared to non-metastatic gastric cancer tissues. MiR-199a positively regulated gastric cancer cell proliferation, migration and invasion. Further studies showed that mitogen-activated protein kinase kinase kinase 11 was significantly down-regulated by miR-199a at the post-transcriptional level and, the level of miR-199a expression in gastric cancer significantly correlated with clinical progression. These findings suggested miR-199a promoted proliferation and metastasis of gastric cancer cells through a regulatory pathway in gastric cancer that has yet to be described. miR-199a may be useful as a new potential therapeutic target for gastric cancer.
小非编码 microRNAs（miRNAs）已被证明在肿瘤增殖和转移中发挥重要作用。然而，它们在胃癌增殖和转移中的功能和机制尚未阐明。在这里，我们研究了 miRNA-199a 与胃癌增殖和转移的关系。通过实时逆转录（RT）-聚合酶链反应，我们发现与正常胃组织相比，miR-199a 在胃癌中高度表达，与非转移性胃癌组织相比，miR-199a 在转移性胃癌组织中高表达。miR-199a 正向调节胃癌细胞增殖、迁移和侵袭。进一步的研究表明，miR-199a 在转录后水平显著下调丝裂原活化蛋白激酶激酶激酶 11 的表达，并且胃癌中 miR-199a 的表达水平与临床进展显著相关。这些发现表明，miR-199a 通过尚未描述的胃癌调控途径促进胃癌细胞的增殖和转移。miR-199a 可能作为一种新的潜在治疗靶点用于胃癌。
