Lampson L A
Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115.
J Immunol. 1990 Jan 15;144(2):512-20.
mAb were used in an immunocytochemical assay to examine beta 2-microglobulin (b2-m) and class I MHC expression in human neuroblastoma cell lines. In lines with weak class I expression among the whole population, under ordinary assay conditions, strong b2-m and class I expression were concentrated in a small subpopulation. In positive cells, Ag was not restricted to any part of the cell body or processes. Strong expression was not required for establishment of any morphologic form or any type of cell contact. These findings complement studies in other experimental systems, where a nonimmunologic role for class I or b2-m in neural cell growth was not revealed. When the microscopic assay was modified to reveal Ag within the internal membrane system, b2-m was detected in every neuroblastoma cell. Most often, the Ag appeared as a ring around the nucleus, or in a punctate distribution in the juxtanuclear area. Internal expression of HLA chains and class I molecules was more difficult to detect, possibly reflecting a normal excess of b2-m. These findings increase understanding of MHC regulation in neural cell lines. They provide the technical and conceptual background for examination of internal MHC Ag in neural tissue.
单克隆抗体（mAb）用于免疫细胞化学分析，以检测人神经母细胞瘤细胞系中β2-微球蛋白（b2-m）和I类主要组织相容性复合体（MHC）的表达。在整个人群中I类表达较弱的细胞系中，在普通检测条件下，强b2-m和I类表达集中在一个小的亚群中。在阳性细胞中，抗原并不局限于细胞体或突起的任何部分。强表达对于任何形态形式或任何类型的细胞接触的建立都不是必需的。这些发现补充了其他实验系统中的研究，在那些研究中未揭示I类或b2-m在神经细胞生长中的非免疫作用。当显微镜检测方法被改进以揭示内膜系统内的抗原时，在每个神经母细胞瘤细胞中都检测到了b2-m。最常见的情况是，抗原表现为围绕细胞核的环状，或在核周区域呈点状分布。HLA链和I类分子的胞内表达更难检测到，这可能反映了b2-m正常情况下的过量。这些发现增进了对神经细胞系中MHC调节的理解。它们为检测神经组织中的胞内MHC抗原提供了技术和概念背景。
