Division of Hematology/Oncology, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Semin Thromb Hemost. 2010 Nov;36(8):865-75. doi: 10.1055/s-0030-1267040. Epub 2010 Nov 3.
Tissue factor (TF) is a transmembrane protein that initiates coagulation following contact with factor VII/VIIa. Recent experimental evidence, in particular from animal models, suggests an important role for circulating TF in thrombosis. This has led to a growing interest in the measurement of TF in whole blood and in cell-free plasma, where functionally active TF is carried on cell-derived microparticles. In this review, we address the range of assays for measuring circulating TF antigen and activity that have been published. We comment on some of the crucial preanalytical and analytical variables that influence the results and their interpretation.
组织因子（TF）是一种跨膜蛋白，在与因子 VII/VIIa 接触后启动凝血。最近的实验证据，特别是来自动物模型的证据，表明循环 TF 在血栓形成中起着重要作用。这导致人们对全血和无细胞血浆中 TF 的测量越来越感兴趣，在无细胞血浆中，功能活跃的 TF 存在于细胞衍生的微粒上。在这篇综述中，我们讨论了已发表的用于测量循环 TF 抗原和活性的一系列检测方法。我们还评论了一些影响结果及其解释的关键分析前和分析变量。
