Pan Hong-ying, Sun Hong-yun, Chen Cui-rong, Chen Qun-wei, Xu Jing, Ye Rong-xia, Lou Guo-qiang, Lu De-rong
The 4th Department of Internal Medicine, Hangzhou Sixth Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310014, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2010 Oct;32(5):557-60. doi: 10.3881/j.issn.1000-503X.2010.05.017.
To evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of spontaneous bacterial peritonitis (SBP).
16S rRNA gene from bacterial DNA in ascites was determined by quantitative fluorescent polymerase chain reaction (PCR) in 76 patients with suspected SBP and 6 patients with non-infectious ascites. The results were compared with those obtained from bacterial culture.
The positive rate of SBP was 22.4% among patients detected with ascitic bacterial 16S rRNA gene determination-based quantitative fluorescent PCR, which was significantly higher than that (7.9%) in patients only received bacterial culture (P<0.05). In addition,in 6 patients with non-infectious ascites,both the 16S rRNA gene determination-based quantitative fluorescent PCR and bacterial culture showed negative results.
16S rRNA gene determination-based quantitative fluorescent PCR can be an effective tool for the rapid diagnosis of SBP. It is more sensitive than the bacterial culture.
评估腹水细菌16S rRNA基因检测在自发性细菌性腹膜炎（SBP）快速诊断中的价值。
采用定量荧光聚合酶链反应（PCR）检测76例疑似SBP患者及6例非感染性腹水患者腹水中细菌DNA的16S rRNA基因，并将结果与细菌培养结果进行比较。
基于腹水细菌16S rRNA基因检测的定量荧光PCR检测SBP患者的阳性率为22.4%，显著高于仅进行细菌培养患者的阳性率（7.9%）（P<0.05）。此外，6例非感染性腹水患者基于16S rRNA基因检测的定量荧光PCR及细菌培养结果均为阴性。
基于16S rRNA基因检测的定量荧光PCR可作为SBP快速诊断的有效工具，其比细菌培养更敏感。
