Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA.
Bioresour Technol. 2011 Feb;102(3):2255-64. doi: 10.1016/j.biortech.2010.10.035. Epub 2010 Oct 14.
The biomethane potential and biodegradability of an array of substrates with highly heterogeneous characteristics, including mono- and co-digestion samples with dairy manure, was determined using the biochemical methane potential (BMP) assay. In addition, the ability of two theoretical methods to estimate the biomethane potential of substrates and the influence of biodegradability was evaluated. The results of about 175 individual BMP assays indicate that substrates rich in lipids and easily-degradable carbohydrates yield the highest methane potential, while more recalcitrant substrates with a high lignocellulosic fraction have the lowest. Co-digestion of dairy manure with easily-degradable substrates increases the specific methane yields when compared to manure-only digestion. Additionally, biomethane potential of some co-digestion mixtures suggested synergistic activity. Evaluated theoretical methods consistently over-estimated experimentally-obtained methane yields when substrate biodegradability was not accounted. Upon correcting the results of theoretical methods with observed biodegradability data, an agreement greater than 90% was achieved.
采用生化甲烷潜能（BMP）测定法，对具有高度异质性特征的一系列基质（包括单独消化和混合消化的奶制品废物样本）的生物甲烷潜能和可生物降解性进行了测定。此外，还评估了两种理论方法估计基质生物甲烷潜能的能力及其对可生物降解性的影响。大约 175 项单独 BMP 测定的结果表明，富含脂质和易降解碳水化合物的基质产生的甲烷潜能最高，而木质纤维素含量较高、更难降解的基质则最低。与仅消化奶制品相比，奶制品废物与易降解基质的混合消化增加了特定甲烷的产率。此外，一些混合消化混合物的生物甲烷潜能表明存在协同作用。当未考虑基质可生物降解性时，评估的理论方法会一直高估实验获得的甲烷产率。在用观察到的可生物降解性数据校正理论方法的结果后，达成了大于 90%的一致性。
