Agriculture and Agri-Food Canada (AAFC), Lethbridge Research Centre, P.O. Box 3000, Lethbridge, Alberta, Canada.
Bioresour Technol. 2010 Aug;101(15):5780-5. doi: 10.1016/j.biortech.2010.02.077. Epub 2010 Mar 23.
Biogas production from anaerobic digestion (AD) of specified risk materials (SRM) co-digested with cattle manure was assessed in a 3 x 2 factorial design. SRM replaced manure at 0 (control), 10% or 25% (w/w) as the substrate fed to six 2-L biodigesters maintained at 37 degrees C or 55 degrees C. Digesters were fed substrate (30 g L(-1) total volatile solids) at 6-d intervals for 90 d, with a retention time of 30 d. Keratin (<20mg) was added to each digester to model the degradation of beta-sheet rich proteins. Methane production was measured daily, and effluent was collected at feeding to monitor SRM degradation using real-time PCR analysis of bovine-specific DNA fragments. Compared with control, methane production increased by 83% or 161% (P<0.05) with 10% or 25% SRM at 37 degrees C, and by 45% and 87%, respectively, at 55 degrees C (P<0.05). Bovine DNA degradation over 6d was higher (P<0.05) at 37 degrees C as compared to 55 degrees C. Dry matter degradation of keratin at 37 degrees C decreased with increasing SRM concentration (P<0.05), whereas at 55 degrees C no difference between treatments was observed (P>0.05). Inclusion of SRM increases the production of methane during the anaerobic digestion of manure and may offer a means of deriving economic value from the disposal of SRM.
采用 3×2 析因设计，评估了与牛粪共消化的特定风险物质（SRM）进行厌氧消化（AD）产生沼气的情况。SRM 取代了 0（对照）、10%或 25%（按干物质计）的粪便作为底物，分别喂给 6 个 2-L 生物消化器，温度保持在 37°C 或 55°C。消化器以 6d 的间隔进料（总挥发性固体 30g/L），停留时间为 30d。向每个消化器中添加角蛋白（<20mg），以模拟富含β-折叠蛋白质的降解。每天测量甲烷产量，并在进料时收集流出物，通过牛特异性 DNA 片段的实时 PCR 分析监测 SRM 降解。与对照相比，37°C 时添加 10%或 25%的 SRM，甲烷产量分别增加了 83%或 161%（P<0.05），而 55°C 时分别增加了 45%和 87%（P<0.05）。与 55°C 相比，37°C 时 6d 内牛 DNA 的降解更高（P<0.05）。37°C 时，角蛋白的干物质降解随 SRM 浓度的增加而降低（P<0.05），而 55°C 时处理之间无差异（P>0.05）。SRM 的包含增加了粪便厌氧消化过程中甲烷的产生，并且可能为从 SRM 的处理中获得经济价值提供了一种手段。
