细胞穿透肽介导的寡核苷酸递送的细胞内化途径的表征

Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery.

作者信息

Guterstam Peter, Andaloussi Samir E L, Langel Ulo

机构信息

Department of Neurochemistry, Stockholm University, Stockholm, Sweden.

出版信息

Methods Mol Biol. 2011;683:219-30. doi: 10.1007/978-1-60761-919-2_16.

DOI:10.1007/978-1-60761-919-2_16

PMID:21053133

Abstract

The methods for evaluating internalization pathways of cellular CPP-mediated ON delivery utilizing a pre-mRNA splice correction assay and fluorescence-based quantification are described. Examples for characterization of CPP uptake routes, employing various endocytosis inhibitors, and special treatment conditions are demonstrated. The methods are developed to characterize cellular delivery of pre-mRNA splice switching peptide nucleic acids conjugated to CPPs by disulfide bond.

摘要

描述了利用前体mRNA剪接校正试验和基于荧光的定量方法评估细胞穿透肽（CPP）介导的寡核苷酸（ON）递送的内化途径。展示了采用各种内吞作用抑制剂和特殊处理条件来表征CPP摄取途径的实例。开发这些方法是为了表征通过二硫键与CPP偶联的前体mRNA剪接转换肽核酸的细胞递送。