采血及抗凝剂对凝血活性、血液学数据及某些流变学测量的影响。
Influence of blood withdrawal and anticoagulant on clotting activity, hematologic data, and certain rheologic measurements.
作者信息
Reinhart W H, Haeberli A, Stark J, Straub P W
机构信息
Department of Internal Medicine, University of Berne, Inselspital, Switzerland.
出版信息
J Lab Clin Med. 1990 Jan;115(1):98-103.
The influence of blood withdrawal (vacuum or slow aspiration) and anticoagulants (ethylenediaminetetraacetic acid [EDTA]; heparin; citrate; a mixture of citrate, theophylline, adenosine, and dipyridamole [CTAD]; and D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone [PPACK]) on clotting activity, hematologic data, and rheologic measurements (whole blood and plasma viscosity, red cell filtration in one study) were investigated. No difference was found between the two blood withdrawal techniques on the basis of the affected measurements. EDTA appeared to be the best anticoagulant with regard to blood cell preservation and showed the lowest whole blood viscosity over a wide range of shear rates (0.1 to 87.0 sec-1). PPACK was the most potent inhibitor of clotting activity as monitored by fibrinopeptide A concentration. The results suggest that EDTA is probably a reasonable choice for rheologic studies of whole blood and should be combined with PPACK when plasma properties are studied.
研究了采血方式（真空采血或缓慢抽吸）和抗凝剂（乙二胺四乙酸[EDTA]；肝素；柠檬酸盐；柠檬酸盐、茶碱、腺苷和双嘧达莫的混合物[CTAD]；以及D-苯丙氨酰-L-脯氨酰-L-精氨酸氯甲基酮[PPACK]）对凝血活性、血液学数据和流变学测量指标（全血和血浆粘度，一项研究中的红细胞滤过率）的影响。基于所测量的指标，未发现两种采血技术之间存在差异。就血细胞保存而言，EDTA似乎是最佳抗凝剂，并且在很宽的剪切速率范围（0.1至87.0秒-1）内显示出最低的全血粘度。通过纤维蛋白肽A浓度监测发现，PPACK是最有效的凝血活性抑制剂。结果表明，EDTA可能是全血流变学研究的合理选择，而在研究血浆特性时应将其与PPACK联合使用。
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