关于用于检测与光动力疗法相关的活性氧和氮物种的荧光探针的应用。
On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy.
机构信息
Wayne State University School of Medicine, Cancer Biology Program, Detroit, Michigan, 48201, USA.
出版信息
J Biomed Opt. 2010 Sep-Oct;15(5):051605. doi: 10.1117/1.3484258.
Abstract
Fluorescent probes are frequently employed for the detection of different reactive oxygen and nitrogen species formed during the irradiation of photosensitized cells and tissues. Investigators often interpret the results in terms of information provided with the different probes without examining specificity or determinants of fluorogenic reactions. We examine five fluorescent probes in a cell-free system: reduced 2',7'-dichlorofluorescein, dihydroethidine, dihydrorhodamine, 3'-(p aminophenyl) fluorescein (APF), and 4',5'-diaminofluorescein. Of these, only APF demonstrates a high degree of specificity for a single reactive species. There is a substantial influence of peroxidase activity on all fluorogenic interactions. The fluorescence of the photosensitizing agent also must be taken into account in evaluating results.
摘要
荧光探针常用于检测光敏细胞和组织辐照过程中形成的不同活性氧和氮物种。研究人员经常根据不同探针提供的信息来解释结果，而不检查荧光反应的特异性或决定因素。我们在无细胞体系中检查了五种荧光探针：还原的 2'，7'-二氯荧光素、二氢乙啶、二氢罗丹明、3'-(对氨基苯基)荧光素（APF）和 4'，5'-二氨基荧光素。其中，只有 APF 对单一反应物种表现出高度特异性。过氧化物酶活性对所有荧光反应都有很大的影响。在评估结果时，还必须考虑到光敏剂的荧光。
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