Xu Kehua, Liu Xia, Tang Bo
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.
Chembiochem. 2007 Mar 5;8(4):453-8. doi: 10.1002/cbic.200600392.
4',9'-Bis(diphenylphosphinyl)naphthofluorescein (PNF-1) has been designed and synthesized as a highly selective, sensitive, cell-permeable, red fluorescent probe for detecting O(2) (.-) in biological systems. The design strategy for the probe is based on the nucleophilic mechanism of O(2) (.-) to mediate deprotection of the probe to naphthofluorescein, the emission spectrum of which is just in the spectral region of low background fluorescence interference in biological systems. Upon treatment with O(2) (.-), the probe exhibits a strong fluorescence response and high selectivity for O(2) (.-), rather than other reactive oxygen species or biological compounds. A linear calibration curve for PNF-1 showed a detection limit of 0.1 nM O(2) (.-). This new type of fluorescent probe allows nanomolar changes in O(2) (.-) concentrations in living cells to be detected by confocal microscopy.
4',9'-双(二苯基膦酰基)萘荧光素(PNF-1)已被设计并合成,作为一种用于检测生物系统中O(2) (.-)的高选择性、高灵敏度、细胞可渗透的红色荧光探针。该探针的设计策略基于O(2) (.-)的亲核机制,以介导探针脱保护生成萘荧光素,其发射光谱恰好在生物系统中低背景荧光干扰的光谱区域。在用O(2) (.-)处理后,该探针表现出对O(2) (.-)的强荧光响应和高选择性,而非对其他活性氧或生物化合物。PNF-1的线性校准曲线显示其对O(2) (.-)的检测限为0.1 nM。这种新型荧光探针能够通过共聚焦显微镜检测活细胞中O(2) (.-)浓度的纳摩尔级变化。