Department of Radiology, Division of Diagnostic Oncology at the Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, 1066 CX Amsterdam, The Netherlands.
J Biomed Opt. 2010 Sep-Oct;15(5):056021. doi: 10.1117/1.3503955.
Accurate tumor excision is crucial in the locoregional treatment of cancer, and for this purpose, surgeons often rely on guide wires or radioactive markers for guidance toward the lesion. Further improvement may be obtained by adding optical guidance to currently used methods, in the form of intra-operative fluorescence imaging. To achieve such a multimodal approach, we have generated markers that can be used in a pre-, intra-, and post-operative setting, based on a cocktail of a dual-emissive inorganic dye, lipids, and pertechnetate. Phantom experiments demonstrate that these seeds can be placed accurately around a surrogate tumor using ultrasound. Three-dimensional bracketing provides delineation of the entire lesion. Combined with the multimodal nature, this provides the opportunity to predetermine the resection margins by validating the placement accuracy using multiple imaging modalities (namely, x ray, MRI, SPECT/CT, and ultrasound). The dual-emissive fluorescent properties of the dye provide the unique opportunity to intra-operatively estimate the depth of the seed in the tissue via multispectral imaging: emission green λmax=520 nm≤5 mm penetration versus emission red λmax=660 nm≤12 mm penetration. By using particles with different colors, the original geographic orientation of the excised tissue can be determined.
准确的肿瘤切除在癌症的局部治疗中至关重要，为此，外科医生通常依靠导丝或放射性标记物来引导病变部位。通过向目前使用的方法中添加光学引导，可以进一步改进，即术中荧光成像。为了实现这种多模态方法，我们已经生成了可以在术前、术中和术后使用的标记物，这些标记物基于双发射无机染料、脂质和高锝酸盐的混合物。体模实验表明，这些种子可以使用超声准确地放置在替代肿瘤周围。三维包围提供了整个病变的描绘。结合多模态特性，这为通过使用多种成像方式（即 X 射线、MRI、SPECT/CT 和超声）验证放置准确性来预先确定切除边界提供了机会。染料的双发射荧光特性提供了通过多光谱成像术中估计种子在组织中深度的独特机会：发射绿光 λmax=520nm≤5mm 穿透，发射红光 λmax=660nm≤12mm 穿透。通过使用不同颜色的粒子，可以确定切除组织的原始地理方向。
