D'Souza D, Das B C, Thomas I M
Department of Anatomy, St. John's Medical College, Bangalore, India.
Mutat Res. 1990 Feb;240(2):101-7. doi: 10.1016/0165-1218(90)90013-r.
The effects of a bifunctional alkylating agent mitomycin C (MMC), an effective inducer of chromosome aberrations and sister-chromatid exchanges (SCEs), have been studied in untreated leprosy patients. This was done to study the mutagen sensitivity of the leprosy patients. The frequency of chromosomal aberrations induced by MMC (conc. 0.01 microgram/ml) was 2.5% in controls, 3.6% in paucibacillary (PB), and 6.8% in multibacillary (MB) patients. The difference in the frequency of MMC-induced chromosome aberrations between the 3 groups studied was highly significant (p less than 0.01). Cultures grown with MMC showed the frequency of SCEs/cell to be 12.70 +/- 1.19 in controls, 19.97 +/- 3.51 in PB, and 29.66 +/- 5.92 in MB patients. The differences in the frequency of MMC-induced SCEs between the 3 groups were found to be highly significant (p less than 0.01). The enhanced frequencies of spontaneous and MMC-induced chromosome aberrations and SCEs observed in PB and MB patients indicate a clear differential mutagen sensitivity between PB and MB patients who are known to have different immunological status and thereby differ in the severity of the disease.
在未经治疗的麻风病患者中研究了双功能烷化剂丝裂霉素C（MMC）的作用，MMC是一种有效的染色体畸变和姐妹染色单体交换（SCE）诱导剂。这样做是为了研究麻风病患者的诱变敏感性。MMC（浓度0.01微克/毫升）诱导的染色体畸变频率在对照组中为2.5%，少菌型（PB）患者中为3.6%，多菌型（MB）患者中为6.8%。所研究的3组之间MMC诱导的染色体畸变频率差异具有高度显著性（p<0.01）。用MMC培养的细胞显示，对照组中每个细胞的SCE频率为12.70±1.19，PB患者中为19.97±3.51，MB患者中为29.66±5.92。发现3组之间MMC诱导的SCE频率差异具有高度显著性（p<0.01）。在PB和MB患者中观察到的自发和MMC诱导的染色体畸变及SCE频率增加表明，已知具有不同免疫状态且疾病严重程度不同的PB和MB患者之间存在明显的诱变敏感性差异。
