You Shu-yuan, Han An-jia, Zhang Fen-fen, Song Yu-lan, Liang Ying-jie, Dong Yu
Department of Pathology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Zhonghua Bing Li Xue Za Zhi. 2010 Aug;39(8):537-41.
To investigate the contribution of latent membrane protein (LMP)1 to nasopharyngeal carcinogenesis via Wnt/β-catenin signal pathway.
The recombinant plasmid pHA2-LMP1 was constructed; immunofluorescence staining, Dual-Luciferase Reporter Assay, Western blot and immunohistochemistry staining were used to study the effect of LMP1 on the transcriptional activity and expression of β-catenin.
(1) Abnormal expression of β-catenin was obtained in 38 cases (50.7%, 38/75), LMP1 expression was obtained in 38 cases (50.7%, 38/75). There was significantly positive correlation between LMP1 expression and abnormal expression of β-catenin in nasopharyngeal carcinoma tissue (P = 0.008). (2) The expression of β-catenin in nuclei of NPC cell line CNE1 and CNE2 transfected with pHA2-LMP1 plasmid dramatically increased, and the expression was remarkable in poorly-differentiated NPC cell line CNE2 than that of well-differentiated CNE1 cells. (3) LMP1 expression dramatically increased the transcriptional activity of β-catenin in CNE1 and CNE2 cells transfected with pHA2-LMP1 and was in a time-dependent. The transcriptional activity of β-catenin was higher in poorly-defferentiated cell line CNE2 than that of well-differentiated NPC cell line CNE1. (4) LMP1 expression did not affect the total protein expression level of β-catenin in both CNE1 and CNE2 cell lines.
EB virus-encoded LMP1 may be involved in the pathogenesis of NPC via β-catenin signal pathway.
通过Wnt/β-连环蛋白信号通路研究潜伏膜蛋白（LMP）1在鼻咽癌发生中的作用。
构建重组质粒pHA2-LMP1；采用免疫荧光染色、双荧光素酶报告基因检测、蛋白质免疫印迹法和免疫组织化学染色研究LMP1对β-连环蛋白转录活性和表达的影响。
（1）75例鼻咽癌组织中38例（50.7%，38/75）β-连环蛋白表达异常，38例（50.7%，38/75）有LMP1表达。鼻咽癌组织中LMP1表达与β-连环蛋白异常表达呈显著正相关（P = 0.008）。（2）转染pHA2-LMP1质粒的鼻咽癌细胞系CNE1和CNE2细胞核中β-连环蛋白表达显著增加，且在低分化鼻咽癌细胞系CNE2中的表达明显高于高分化CNE1细胞。（3）LMP1表达显著增加了转染pHA2-LMP1的CNE1和CNE2细胞中β-连环蛋白的转录活性，且呈时间依赖性。低分化细胞系CNE2中β-连环蛋白的转录活性高于高分化鼻咽癌细胞系CNE1。（4）LMP1表达不影响CNE1和CNE2细胞系中β-连环蛋白的总蛋白表达水平。
EB病毒编码的LMP1可能通过β-连环蛋白信号通路参与鼻咽癌的发病机制。
