小鼠肝脏衍生抑制蛋白的特性分析

Characterization of murine liver-derived inhibitory protein.

作者信息

Wang S R, Huang M H, Chang K L, Yu C L, Chiang B N, Han S H

机构信息

Department of Medicine, Veterans General Hospital, Taiwan, ROC.

出版信息

Scand J Immunol. 1990 Jan;31(1):85-90. doi: 10.1111/j.1365-3083.1990.tb02746.x.

DOI:10.1111/j.1365-3083.1990.tb02746.x

PMID:2105527

Abstract

Murine liver-derived inhibitory protein (LIP) capable of inhibiting human lymphocyte proliferation was highly purified from liver extract. Its molecular weight determined by gel filtration and SDS-PAGE was 105,000 and 38,400 respectively. LIP moved electrophoretically at the gamma-globulin region. Its activity in inhibiting lymphocyte proliferation was temperature-stable up to 60 degrees C, and pH-stable between 4 and 11. It was not cytotoxic to lymphocytes as shown in 51Cr-release experiments. The purified LIP possessed arginase activity.

摘要

从小鼠肝脏提取物中高度纯化出能够抑制人淋巴细胞增殖的小鼠肝脏衍生抑制蛋白（LIP）。通过凝胶过滤和SDS-PAGE测定其分子量分别为105,000和38,400。LIP在γ-球蛋白区域进行电泳迁移。其抑制淋巴细胞增殖的活性在高达60摄氏度时对温度稳定，在pH值4至11之间对pH稳定。如51Cr释放实验所示，它对淋巴细胞无细胞毒性。纯化的LIP具有精氨酸酶活性。