[Study on the biological characteristics of adipose stem cells derived from renal adipose capsule cultured in vitro].

AIM

To investigate the culture conditions and biological characteristics of the adipose-derived stem cells (ADSCs) isolated from renal adipose capsule so as to find a better source of stem cells for the treatment of kidney disease.

METHODS

Renal adipose capsules and groin adipose tissues were taken from rats. The isolated and cultured ADSCs from the two regions were compared.When approximately 80% cell fusion were achieved, cell passage was established. To observe cell morphology and proliferation, we adopted MTT colorimetry to record cell growth curve, and utilized FCM to detect the expressions of cell surface antigen.

RESULTS

(1) The primary ADSCs showed that the number of ADSCs isolated from the renal adipose capsules significantly exceed that from the groin adipose tissues at the same quality. About 80% ADSCs derived from the renal adipose capsules fused in 5-6 days, while the ADSCs from the inguinal fat tissues needed 10-12 days approximately. Both were spindle-like or fibroblast-like in shape. (2) The analysis of the growth curve showed that the proliferation of ADSCs from renal fat capsule was faster than that from inguinal fat tissues. (3) FCM showed that CD29 antigen expression rates of ADSCs from both parts exceeded 99%. The expression rates of the renal ADSCs for CD44 was 52.92%, while the expression rate of the groin ADSCs was 75.41%. The marking antigens of the endothelial cells for CD31 were negative.

CONCLUSION

Compared with the ADSCs taken from the inguinal region, the renal ADSCs has the advantages of greater cell culture number and faster proliferation, which may become a new source of stem cells to treat the renal disease.