Ma Ying, Xu Xiao-guang, Huang Hai-yan, Li Qi, Song Chao-jun, Shi Bing-yi, Jin Bo-quan
Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Nov;26(11):1140-2.
To establish ELISA method for quantitate the concentration of cystatin C (cys C) and to monitor the renal function of patients before and after renal transplantation.
Hybridomas secreting monoclonal antibodies (mAbs) against human cys C were produced and sandwich ELISA kit for quantitatively detecting cys C was established. Then the concentrations of serum cystatin C (Scys C) and urine cystatin C (Ucys C) from normal controls and 23 patients undergoing renal transplantation were detected and their relationship with serum creatinine (SCR) was analyzed.
Seven hybridomas secreting anti-cys C mAbs were obtained. The sensitivity of the established ELISA kit reached 0.1 μg/L. The concentrations of Scys C and Ucys C of normal healthy controls were in accordance with other report. High correlations between Scys C or Ucys C and the level of SCR were observed (P<0.01). Rapid decline of Scys C and Ucys C concentrations was consistent with the decrease of SCR in the patients with normal course (NC) recovery after renal transplantation. However, Ucys C kept higher level within two weeks after the operation in patients with AR until the day 21. In patients with DGF, higher levels of Scys C, Ucys C and SCR were sustained within four weeks after renal transplantation.
The sensitive ELISA kit for detection of cys C has been established. Importantly, there are the persistently high levels of Scys C and Ucys C in patients with AR or DGF, which can be used as a novel indicator for monitoring renal function after renal transplantation.
建立酶联免疫吸附测定（ELISA）法以定量检测胱抑素C（cys C）的浓度，并监测肾移植患者术前及术后的肾功能。
制备分泌抗人cys C单克隆抗体（mAb）的杂交瘤细胞，并建立用于定量检测cys C的夹心ELISA试剂盒。然后检测正常对照组和23例肾移植患者的血清胱抑素C（Scys C）和尿胱抑素C（Ucys C）浓度，并分析它们与血清肌酐（SCR）的关系。
获得了7株分泌抗cys C mAb的杂交瘤细胞。所建立的ELISA试剂盒灵敏度达到0.1μg/L。正常健康对照组的Scys C和Ucys C浓度与其他报道一致。观察到Scys C或Ucys C与SCR水平之间存在高度相关性（P<0.01）。在肾移植后病程正常（NC）恢复的患者中，Scys C和Ucys C浓度迅速下降，与SCR的降低一致。然而，急性排斥反应（AR）患者术后两周内Ucys C水平持续较高，直至第21天。在移植肾功能延迟恢复（DGF）患者中，肾移植后四周内Scys C、Ucys C和SCR水平持续较高。
已建立了用于检测cys C的灵敏ELISA试剂盒。重要的是，AR或DGF患者中Scys C和Ucys C持续高水平，可作为监测肾移植后肾功能的新指标。
