Takada A, Takada Y, Urano T, Sakakibara K, Rydzewski A
Department of Physiology, Hamamatsu University, School of Medicine, Shizuoka-ken, Japan.
Thromb Res. 1990 Jan 1;57(1):13-20. doi: 10.1016/0049-3848(90)90191-e.
Blood was taken from healthy 15 males and 10 females at 9:30 h, 10:00 h and 12:30 h. The euglobulin fraction was prepared and clotted by the addition of human thrombin. The clot lysis time shortened significantly from 9:30 h to 10:00 h (p less than 0.01) and further to 12:30 h (p less than 0.01). Plasma levels of tissue plasminogen activator (t-PA) antigens did not change from 9:30 h to 10:00 h, but slightly and significantly to 12:30 h (p less than 0.01). Plasma levels of free plasminogen activator inhibitor-1 (PAI-1) and complex of t-PA-PAI-1 decreased from 9:30 h to 10:00 h (p less than 0.02) and to 12:30 h (p less than 0.01). Plasma levels of total PAI-1 (free plus complex) decreased from 9:30 h to 10:00 h (p less than 0.01) and to 12:30 h (p less than 0.01). These results suggest that a major factor contributing to the enhanced fibrinolytic activity of the euglobulin fraction may be a level of PAI-1 (free and total).
在上午9:30、10:00和12:30采集了15名健康男性和10名健康女性的血液。制备优球蛋白组分，并通过添加人凝血酶使其凝固。从9:30到10:00，凝块溶解时间显著缩短（p<0.01），到12:30进一步缩短（p<0.01）。组织纤溶酶原激活物（t-PA）抗原的血浆水平从9:30到10:00没有变化，但到12:30有轻微且显著的变化（p<0.01）。游离纤溶酶原激活物抑制剂-1（PAI-1）和t-PA-PAI-1复合物的血浆水平从9:30到10:00降低（p<0.02），到12:30也降低（p<0.01）。总PAI-1（游离加复合物）的血浆水平从9:30到10:00降低（p<0.01），到12:30也降低（p<0.01）。这些结果表明，导致优球蛋白组分纤溶活性增强的一个主要因素可能是PAI-1（游离和总）的水平。
