虾青素体内处理诱导糖尿病大鼠中性粒细胞中活性氧的产生。
ROS production in neutrophils from alloxan-induced diabetic rats treated in vivo with astaxanthin.
机构信息
Postgraduate Program, Human Movement Sciences Institute of Physical Activity and Sport Sciences, Cruzeiro do Sul University, São Paulo, SP, Brazil, 01506-000.
出版信息
Int Immunopharmacol. 2011 Jan;11(1):103-9. doi: 10.1016/j.intimp.2010.10.013. Epub 2010 Nov 4.
BACKGROUND
Astaxanthin (ASTA) is a carotenoid which has powerful antioxidant, anti-tumor, anti-diabetic, anti-inflammatory and cardioprotective properties. The present study investigated the effect of daily ASTA intake on oxidative stress and the functional properties of neutrophils from alloxan-induced diabetic rats.
METHODS
Neutrophils isolated from ASTA-fed rats (30days, 20mg ASTA/kg of body weight - BW) induced to diabetes by alloxan treatment (i.p. 75mg/BW) were assessed by: production of superoxide and hydrogen peroxide, nitric oxide, basal calcium release, oxidative damage (TBARS and carbonyls content), and activities of major antioxidant enzymes.
RESULTS
Our results show that diabetes promotes a significant oxidative stress in neutrophils. The production of superoxide was significantly increased in neutrophils from diabetic rats and treatment with ASTA was not effective in reducing superoxide levels. At the same time, a reduction in the activity of total superoxide dismutase enzyme was observed, which was not restored after treatment with ASTA. At resting conditions, neutrophils have a higher basal production of hydrogen peroxide, which is enhanced following PMA-stimulation. Treatment with ASTA does not restore values to the basal levels. The indicators of oxidative damage to biomolecules showed that diabetic rats significantly increased the lipid and protein damage, but this change was reversed after treatment with ASTA.
CONCLUSION
Our results show that diabetes condition promotes a marked oxidative stress in neutrophils and treatment with ASTA for 30days at a dose of 20mg/kg of BW partially reverses those deleterious effects.
背景
虾青素（ASTA）是一种类胡萝卜素，具有强大的抗氧化、抗肿瘤、抗糖尿病、抗炎和心脏保护作用。本研究旨在探讨每日 ASTA 摄入对丙烯醛诱导糖尿病大鼠中性粒细胞氧化应激和功能特性的影响。
方法
用丙烯醛（腹腔注射 75mg/kg 体重）处理分离自 ASTA 喂养大鼠（30 天，20mg/kg 体重）的中性粒细胞，评估超氧阴离子和过氧化氢、一氧化氮、基础钙释放、氧化损伤（TBARS 和羰基含量）以及主要抗氧化酶的活性。
结果
研究结果表明，糖尿病会导致中性粒细胞发生显著氧化应激。糖尿病大鼠中性粒细胞中超氧阴离子的产生显著增加，而 ASTA 处理并不能有效降低超氧阴离子水平。同时，观察到总超氧化物歧化酶酶活性降低，ASTA 处理后并未恢复。在静息状态下，中性粒细胞基础产生的过氧化氢水平较高，经 PMA 刺激后进一步增强。ASTA 处理并不能将其恢复到基础水平。生物分子氧化损伤指标表明，糖尿病大鼠的脂质和蛋白质损伤显著增加，但经 ASTA 处理后这种变化得到逆转。
结论
研究结果表明，糖尿病状态会导致中性粒细胞发生明显的氧化应激，而每日 20mg/kg 体重的 ASTA 治疗 30 天可部分逆转这些有害影响。
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