在羟基磷灰石溶胶-凝胶合成膜上形成磷脂双层。

Phospholipid bilayer formation on hydroxyapatite sol-gel synthesized films.

机构信息

Department of Chemical Engineering & Materials Science, University of California, Davis, CA 95616, United States.

出版信息

Colloids Surf B Biointerfaces. 2011 Feb 1;82(2):647-50. doi: 10.1016/j.colsurfb.2010.10.016. Epub 2010 Oct 15.

DOI:10.1016/j.colsurfb.2010.10.016

Abstract

Lipid bilayers supported by porous biomaterials are being explored as models for cell membranes. Hydroxyapatite is a relevant material currently being used extensively for biomedical applications. In this study, hydroxyapatite films produced via a sol-gel chemistry route have been characterized and explored as a scaffolding material for lipid membranes. The hydroxyapatite has been characterized using XRD, SEM, and AFM, followed by vesicle-fusion of lipids characterized by fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) to determine the diffusion coefficient of this system. The HA films produced in this work were found to produce slow lateral diffusion and, in the two-phase lipid systems, some domains were observed. The low lateral diffusion coefficients were believed to be a result of the large undulations present on the hydroxyapatite film surface.

摘要

多孔生物材料支撑的脂质双层膜作为细胞膜模型正在被探索。羟基磷灰石是一种相关的材料，目前正在广泛应用于生物医学领域。在这项研究中，通过溶胶-凝胶化学途径制备的羟基磷灰石薄膜已被表征，并作为脂质膜的支架材料进行了探索。使用 XRD、SEM 和 AFM 对羟基磷灰石进行了表征，然后通过荧光显微镜和荧光漂白后恢复（FRAP）对脂质囊泡融合进行了表征，以确定该体系的扩散系数。本工作中制备的 HA 薄膜被发现具有缓慢的侧向扩散，并且在两相脂质体系中观察到一些域。较低的侧向扩散系数被认为是由于羟基磷灰石薄膜表面存在较大的起伏。