Cancer Epidemiology Branch, Division of Cancer Epidemiology and Management, Research Institute, National Cancer Center, Goyang-si Gyeonggi-do, 410-769, Republic of Korea.
Nutr Res. 2010 Oct;30(10):705-13. doi: 10.1016/j.nutres.2010.09.012.
Disturbances in redox regulation are suggested to be involved in the development of breast cancer. We conducted a hospital-based case-control study to examine the hypothesis that lower plasma antioxidant concentration is related to higher risk of breast cancer and that genetic polymorphism of manganese superoxide dismutase (SOD2) modifies the relationship between breast cancer risk and plasma antioxidant. Genotyping for SOD2 Val16Ala polymorphism was performed by a 5' exonuclease assay, and plasma concentrations of retinol, carotenoids, and tocopherols were determined by high-performance liquid chromatography. Unconditional logistic regression models were used to estimate crude and multivariate odds ratios with a 95% confidence interval. The variant allele frequencies of SOD2 Val16Ala (TC or CC type) were 26% for the control subjects and 23% for the breast cancer patients, and the variant genotype was not a risk factor for breast cancer. Higher plasma retinol concentration was associated with a lower risk, whereas higher plasma β-carotene, α-tocopherol, or γ-tocopherol concentrations were associated with a higher risk of breast cancer. SOD2 CT or CC genotype was associated with lower risk (odds ratio, 0.53; 95% confidence interval, 0.30-0.93; P for interaction = .025) in subjects with low plasma γ-tocopherol concentration. Our findings suggest that the SOD2 Val16Ala variant is not related to the risk of breast cancer in Korean women; however, it may affect the association between plasma γ-tocopherol levels and the risk of breast cancer.
氧化还原调节紊乱被认为与乳腺癌的发展有关。我们进行了一项基于医院的病例对照研究，以检验以下假设：血浆抗氧化剂浓度较低与乳腺癌风险较高有关，而锰超氧化物歧化酶（SOD2）的遗传多态性会改变乳腺癌风险与血浆抗氧化剂之间的关系。通过 5'外切酶分析对 SOD2 Val16Ala 多态性进行基因分型，通过高效液相色谱法测定视黄醇、类胡萝卜素和生育酚的血浆浓度。使用无条件逻辑回归模型估计粗和多变量优势比，并带有 95%置信区间。SOD2 Val16Ala（TC 或 CC 型）的变异等位基因频率为对照组的 26%和乳腺癌患者的 23%，变异基因型不是乳腺癌的危险因素。较高的血浆视黄醇浓度与较低的风险相关，而较高的血浆 β-胡萝卜素、α-生育酚或γ-生育酚浓度与乳腺癌的风险增加相关。SOD2 CT 或 CC 基因型与低血浆γ-生育酚浓度的个体较低的风险相关（比值比，0.53；95%置信区间，0.30-0.93；P 交互作用=0.025）。我们的研究结果表明，SOD2 Val16Ala 变体与韩国女性乳腺癌的风险无关；然而，它可能会影响血浆γ-生育酚水平与乳腺癌风险之间的关联。
