Department of Prosthodontics, School of Stomatology, Capital Medical University, Tian Tan Xi Li No. 4, Beijing 100050, China.
J Biotechnol. 2011 Jan 10;151(1):87-93. doi: 10.1016/j.jbiotec.2010.10.080. Epub 2010 Nov 5.
Fabricating individualized tissue engineering scaffolds based on the three-dimensional shape of patient bone defects is required for the successful clinical application of bone tissue engineering. However, there are currently no reported studies of individualized bone tissue engineering scaffolds that truly reproduce a patient-specific bone defect. We fabricated individualized tissue engineering scaffolds based on alveolar bone defects. The individualized poly(lactide-co-glycolide) and tricalcium phosphate composite scaffolds were custom-made by acquiring the three-dimensional model through computed tomography, which was input into the computer-aided low-temperature deposition manufacturing system. The three-dimensional shape of the fabricated scaffold was identical to the patient-specific alveolar bone defects, with an average macropore diameter of 380 μm, micropore diameters ranging from 3 to 5 μm, and an average porosity of 87.4%. The mechanical properties of the scaffold were similar to adult cancellous bone. Scaffold biocompatibility was confirmed by attachment and proliferation of human bone marrow mesenchymal stem cells. Successful realization of individualized scaffold fabrication will enable clinical application of tissue-engineered bone at an early date.
基于患者骨缺损的三维形状制造个性化组织工程支架对于骨组织工程的成功临床应用是必需的。然而，目前尚无关于真正再现患者特异性骨缺损的个体化骨组织工程支架的报道研究。我们基于牙槽骨缺损制造了个体化组织工程支架。通过计算机断层扫描获取三维模型，然后将其输入计算机辅助低温沉积制造系统，定制个体化的聚（乳酸-共-乙醇酸）和磷酸三钙复合材料支架。所制造的支架的三维形状与特定患者的牙槽骨缺损完全一致，平均大孔直径为 380μm，微孔直径范围为 3 至 5μm，平均孔隙率为 87.4%。支架的机械性能类似于成人松质骨。通过人骨髓间充质干细胞的附着和增殖证实了支架的生物相容性。成功实现个体化支架制造将使组织工程骨能够早日临床应用。
