Matsui Y, Kikuchi A, Kawata M, Kondo J, Teranishi Y, Takai Y
Research Center, Mitsubishi Kasei Corporation, Yokohama, Japan.
Biochem Biophys Res Commun. 1990 Jan 30;166(2):1010-6. doi: 10.1016/0006-291x(90)90911-6.
We have previously purified smg p21 from bovine brain membranes and isolated its cDNA from a bovine brain cDNA library. In the present studies, we have performed extensive screening of the bovine brain cDNA library with the cloned smg p21 cDNA as a probe and isolated another cDNA encoding a protein highly homologous to smg p21. The proteins encoded by the previously and newly isolated cDNAs are designated as smg p21A and -B, respectively. Since the partial amino acid sequences determined previously from the smg p21 purified from bovine brain were identical with the common amino acid sequences between smg p21A and -B, we have further sequenced smg p21 and identified it as smg p21B. We have also further sequenced the smg p21 purified from human platelet membranes and identified it as smg p21B. Amino acid sequence analysis indicates that smg p21A is identical with the rap1A and Krev-1 proteins and smg p21B is identical with the rap1B protein.
我们之前已从牛脑膜中纯化出smg p21，并从牛脑cDNA文库中分离出其cDNA。在本研究中，我们以克隆的smg p21 cDNA为探针，对牛脑cDNA文库进行了广泛筛选，分离出另一个编码与smg p21高度同源蛋白质的cDNA。先前和新分离的cDNA所编码的蛋白质分别命名为smg p21A和smg p21B。由于先前从牛脑纯化的smg p21中确定的部分氨基酸序列与smg p21A和smg p21B之间的共同氨基酸序列相同，我们进一步对smg p21进行了测序，并将其鉴定为smg p21B。我们还进一步对从人血小板膜纯化的smg p21进行了测序，并将其鉴定为smg p21B。氨基酸序列分析表明，smg p21A与rap1A和Krev-1蛋白相同，smg p21B与rap1B蛋白相同。
