Molter Timothy W, McQuaide Sarah C, Holl Mark R, Meldrum Deirdre R, Dragavon Joseph M, Anderson Judith B, Young A Cody, Burgess Lloyd W, Lidstrom Mary E
Department of Electrical Engineering, University of Washington, Seattle, WA 98195-2500 USA (
IEEE Trans Autom Sci Eng. 2008 Jan 1;5(1):32-42. doi: 10.1109/tase.2007.909441.
A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.
本文介绍了一种能够测量单细胞耗氧率的新型系统。实验装置包括一个温度可控的环境舱、蚀刻在玻璃上的微孔阵列以及用于密封微孔中细胞的盖驱动器。每个微孔都包含一个对氧敏感的铂磷光体传感器,用于监测细胞代谢率。定制的自动化软件控制用于氧传感器测量的数字图像数据收集,这些数据通过图像处理程序进行分析,以得出每个微孔内的氧浓度随时间的变化情况。两项概念验证实验得出A549人上皮肺癌细胞的耗氧率测量值分别为5.39和5.27 fmol/分钟/细胞,与已发表的大量A549细胞群体的耗氧率非常匹配。