Chongqing Key Laboratory of Nutrition and Food Safety, Third Military University, Chongqing, China.
Nutr Cancer. 2010;62(8):1128-36. doi: 10.1080/01635581.2010.494821.
Anthocyanins widely present in human diet and have a variety of health effects. This study investigates the anticancer effects of an anthocyanin-rich extract from black rice (AEBR) on breast cancer cells in vitro and in vivo. AEBR reduced the viability of breast cancer cell lines MCF-7 (ER(+), HER2/neu(-)), MDA-MB-231 (ER(-), HER2/neu(-)), and MDA-MB-453 (ER(-), HER2/neu(+)) and induced apoptosis in MDA-MB-453 cells via the intrinsic pathway in vitro by activating caspase cascade, cleaving poly (ADP-ribose) polymerase (PARP), depolarizing mitochondrial membrane potential, and releasing cytochrome C. Oral administration of AEBR (100 mg/kg/day) to BALB/c nude mice bearing MDA-MB-453 cell xenografts significantly suppressed tumor growth and angiogenesis by suppressing the expression of angiogenesis factors MMP-9, MMP-2, and uPA in tumor tissue. Altogether, this study suggests the anticancer effects of AEBR against human breast cancer cells in vitro and in vivo by inducing apoptosis and suppressing angiogenesis.
花色苷广泛存在于人类饮食中，具有多种健康功效。本研究探讨了黑米花色苷提取物（AEBR）对体外和体内乳腺癌细胞的抗癌作用。AEBR 通过激活半胱天冬酶级联反应、切割多聚（ADP-核糖）聚合酶（PARP）、使线粒体膜电位去极化和释放细胞色素 C，降低 MCF-7（ER(+)，HER2/neu(-)）、MDA-MB-231（ER(-)，HER2/neu(-)）和 MDA-MB-453（ER(-)，HER2/neu(+)）乳腺癌细胞系的活力，并诱导 MDA-MB-453 细胞通过内在途径发生细胞凋亡。体内实验结果表明，AEBR（100mg/kg/天）灌胃荷 MDA-MB-453 细胞异种移植瘤的 BALB/c 裸鼠，能显著抑制肿瘤生长和血管生成，其作用机制可能与下调肿瘤组织中血管生成因子 MMP-9、MMP-2 和 uPA 的表达有关。综上所述，本研究提示 AEBR 通过诱导细胞凋亡和抑制血管生成，对体外和体内人乳腺癌细胞具有抗癌作用。
