Ayar-Kayali H, Tarhan L
University of Dokuz Eylül, Faculty of Science and Art, Department of Chemistry, Biochemistry Division, 35150 Buca, Izmir, Turkey.
Prikl Biokhim Mikrobiol. 2010 Sep-Oct;46(5):519-26.
The relationship between tricarboxylic acid (TCA) and glyoxalate cycle and the effect of their metabolites levels on the vancomycin production of Amycolatopsis orientalis were investigated in different concentration of glycerol (2.5-20 g/l). Intracellular glycerol levels increased with respect to increases in glycerol concentrations of the growth medium. Extracellular glycerol levels decreased slowly up to 24 h while uptake rates were increased during 36-48 h for 10 and 15 g/l and during 36-60 h at 20 g/l of glycerol. Intracellular citrate, alpha-ketoglutarate, fumarate levels increased up to 10 g/l glycerol concentration. However, intracellular succinate and malate levels were increased up to 15 g/l glycerol. Extracellular citrate, alpha-ketoglutarate, succinate and malate levels increased with respect to increases in glycerol concentration. The highest alpha-ketoglutarate dehydrogenase activity was determined at 15 g/l glycerol. Isocitrate lyase activity showed a positive correlation with the increases in glycerol concentration of the growth medium. Vancomycin production increased with the increases in glycerol concentration from 5 to 10 g/l. These results showed that A. orientalis grown in glycerol containing medium used glyoxalate shunt actively instead of TCA cycle which supports precursors of many amino acid which are effective on the antibiotic production.
在不同浓度甘油（2.5 - 20 g/l）条件下，研究了东方拟无枝酸菌中三羧酸（TCA）循环和乙醛酸循环之间的关系及其代谢物水平对万古霉素产量的影响。随着生长培养基中甘油浓度的增加，细胞内甘油水平升高。细胞外甘油水平在24小时内缓慢下降，而在甘油浓度为10和15 g/l时，36 - 48小时内摄取速率增加；在甘油浓度为20 g/l时，36 - 60小时内摄取速率增加。细胞内柠檬酸、α - 酮戊二酸、富马酸水平在甘油浓度达到10 g/l时升高。然而，细胞内琥珀酸和苹果酸水平在甘油浓度达到15 g/l时升高。细胞外柠檬酸、α - 酮戊二酸、琥珀酸和苹果酸水平随着甘油浓度的增加而升高。在甘油浓度为15 g/l时，α - 酮戊二酸脱氢酶活性最高。异柠檬酸裂解酶活性与生长培养基中甘油浓度的增加呈正相关。从5到10 g/l，随着甘油浓度的增加，万古霉素产量增加。这些结果表明，在含甘油培养基中生长的东方拟无枝酸菌积极利用乙醛酸支路而非三羧酸循环，三羧酸循环为许多对抗生素生产有效的氨基酸提供前体。
