State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China.
Biomed Mater. 2010 Dec;5(6):065011. doi: 10.1088/1748-6041/5/6/065011. Epub 2010 Nov 9.
Nanofibrous scaffolds have been applied widely in tissue engineering to simulate the nanostructure of natural extracellular matrix (ECM) and promote cell bioactivity. The aim of this study was to design a biocompatible nanofibrous scaffold for blood outgrowth endothelial cells (BOECs) and investigate the interaction between the topography of the nanofibrous scaffold and cell growth. Poly(L-lactic acid) (PLLA) random and aligned nanofibers with a uniform diameter distribution were fabricated by electrospinning. NH(3) plasma etching was used to create a hydrophilic surface on the nanofibers to improve type-I collagen adsorption; the conditions of the NH(3) plasma etching were optimized by XPS and water contact angle analysis. Cell attachment, proliferation, viability, phenotype and morphology of BOECs cultured on type-I collagen-coated PLLA film (col-Film), random fibers (col-RFs) and aligned fibers (col-AFs) were detected over a 7 day culture period. The results showed that collagen-coated PLLA nanofibers improved cell attachment and proliferation; col-AFs induced the directional growth of cells along the aligned nanofibers and enhanced endothelialization. We suggest that col-AFs may be a potential implantable scaffold for vascular tissue engineering.
纳米纤维支架已广泛应用于组织工程中，以模拟天然细胞外基质（ECM）的纳米结构，促进细胞的生物活性。本研究旨在设计一种用于血液衍生内皮细胞（BOECs）的生物相容性纳米纤维支架，并研究纳米纤维支架的形貌与细胞生长之间的相互作用。通过静电纺丝制备了具有均匀直径分布的聚 L-乳酸（PLLA）无规和定向纳米纤维。NH3 等离子体刻蚀用于在纳米纤维上制造亲水表面，以提高 I 型胶原的吸附；通过 XPS 和水接触角分析优化了 NH3 等离子体刻蚀的条件。在 7 天的培养过程中，检测了 BOECs 在 I 型胶原涂覆 PLLA 膜（col-Film）、无规纤维（col-RFs）和定向纤维（col-AFs）上的黏附、增殖、活力、表型和形态。结果表明，胶原涂覆的 PLLA 纳米纤维可提高细胞黏附率和增殖；col-AFs 诱导细胞沿着定向纳米纤维定向生长，并增强内皮化。我们认为 col-AFs 可能是一种有潜力的用于血管组织工程的植入式支架。
