Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan, Republic of China.
Biomed Mater. 2010 Dec;5(6):065012. doi: 10.1088/1748-6041/5/6/065012. Epub 2010 Nov 9.
A variable gene delivery system has been developed based on conjugating chitosan to biotin through a functionalized poly(ethylene glycol) (PEG) spacer, which can be used to further bind different molecules on the outer layer of a polymer/DNA complex by streptavidin (SA)-biotin linkage. In this study, TAT-conjugated SA was used as the model molecule to prove the conjugation function of the prepared complex. In addition, low-molecular-weight poly(ethyleneimine) (PEI) was added into the polymer/DNA complex to increase the transfection efficiency. The results of the luciferase assay show that the transfection efficiency of the prepared complex was significantly correlated with the amount of PEI and was further enhanced when TAT was conjugated to the complex by SA-biotin linkage. Considered to have negligible cytotoxic effects, the variable gene delivery complex prepared in this study would be of considerable potential as carriers for in vitro applications.
已经开发出一种基于通过功能化聚乙二醇 (PEG) 间隔物将壳聚糖偶联到生物素的可变基因传递系统，该系统可用于通过链霉亲和素 (SA)-生物素键合进一步将不同的分子结合到聚合物/DNA 复合物的外层上。在这项研究中，使用 TAT 缀合的 SA 作为模型分子来证明所制备的复合物的缀合功能。此外，将低分子量的聚乙烯亚胺 (PEI) 添加到聚合物/DNA 复合物中以提高转染效率。荧光素酶测定的结果表明，所制备的复合物的转染效率与 PEI 的量显著相关，并且当 TAT 通过 SA-生物素键合连接到复合物时，转染效率进一步增强。由于被认为具有可以忽略的细胞毒性作用，因此本研究中制备的可变基因传递复合物作为体外应用的载体具有相当大的潜力。
