Polymeric structure of a cyclic adenosine 3', 5',-monophosphate dependent protein kinase from the dimorphic fungus Mucor rouXII.

Adenosine 3', 5'-monophosphate (cAMP) dependent protein kinase from yeast cells of Mucor rouxii was partially purified and examined by sedimentation in sucrose density gradients. In the presence of histone and cAMP this procedure allowed the separation of the catalytic moiety from the cAMP binding activity, indicating that the enzyme had dissociated into subunits. The dissociation was accompanied by conversion of the enzyme activity from a cAMP dependent to a cAMP independent form.