Kobusch A B, Bock K W
Institute of Toxicology, University of Tübingen, Federal Republic of Germany.
Biochem Pharmacol. 1990 Feb 1;39(3):555-8. doi: 10.1016/0006-2952(90)90063-q.
To investigate factors influencing cell proliferation, cells are often cultured in serum-free medium. In the present study it is shown that addition of zinc chloride (40 microM) to primary mouse hepatocytes, cultured in Dulbecco's minimal essential medium, markedly enhanced growth factor (EGF)-stimulated [3H]thymidine incorporation into DNA. Treatment of cell cultures with phenobarbital or 3,4,3',4'-tetrachlorobiphenyl (enzyme inducers and tumor promoters in vivo) or with 12-O-tetradecanoylphorbol-13-acetate (the classical skin tumor promoter) further increased EGF-stimulated DNA synthesis. The results emphasize the need to adequately substitute zinc in serum-free cultured cells.
为了研究影响细胞增殖的因素，细胞通常在无血清培养基中培养。本研究表明，在杜尔贝科改良伊格尔培养基中培养的原代小鼠肝细胞中添加氯化锌（40微摩尔），可显著增强生长因子（EGF）刺激的[3H]胸腺嘧啶核苷掺入DNA的能力。用苯巴比妥或3,4,3',4'-四氯联苯（体内的酶诱导剂和肿瘤促进剂）或12-O-十四烷酰佛波醇-13-乙酸酯（经典的皮肤肿瘤促进剂）处理细胞培养物，可进一步增加EGF刺激的DNA合成。结果强调了在无血清培养细胞中充分补充锌的必要性。
