Bakır Saygan Sibel, Yaşar Handan, Eras Zeynep, Cesur Salih, Irmak Hasan, Dilmen Uğur, Demiröz Ali Pekcan
Dr. Zekai Tahir Burak Kadın Sağlığı Eğitim ve Araştırma Hastanesi, Enfeksiyon Kontrol Komitesi, Ankara, Türkiye.
Mikrobiyol Bul. 2010 Jul;44(3):529-31.
This study was aimed to determine the rates of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) carriage in a neonatal intensive care unit in a Training and Research Hospital in Ankara, Turkey. A total of 135 newborns were included in the study. Following 5 days stay in intensive care unit, samples were taken from nose and umbilicus for the detection of MRSA and cultivated in mannitol-salt agar and oxacillin-resistance screening agar (ORSAB), respectively. The samples taken from rectum to screen VRE, were placed onto Enterococcosel agar which contained vancomycin and ceftazidime. The confirmation of methicillin resistance in MRSA suspected isolates was performed by oxacillin and cefoxitin disk diffusion tests according to Clinical Laboratory Standards Institute (CLSI) guidelines. Twenty eight (20.7%) of 135 newborns had nasal MRSA carriage, 30 (22.2%) of 135 had umbilical and 10 (7.4%) had both nasal and umbilical MRSA carriage. No rectal VRE carriage was found among the newborns. As a result, we suggest that periodical MRSA and VRE carriage investigation in the patients hospitalized in neonatal intensive care units is of outmost help to control and prevent nosocomial infections.
本研究旨在确定土耳其安卡拉一家培训与研究医院新生儿重症监护病房中耐甲氧西林金黄色葡萄球菌(MRSA)和耐万古霉素肠球菌(VRE)的携带率。共有135名新生儿纳入本研究。在重症监护病房住院5天后,从鼻腔和脐部分别采集样本以检测MRSA,并分别接种于甘露醇盐琼脂和苯唑西林耐药筛选琼脂(ORSAB)中培养。从直肠采集用于筛查VRE的样本,接种于含万古霉素和头孢他啶的肠球菌琼脂上。根据临床实验室标准协会(CLSI)指南,通过苯唑西林和头孢西丁纸片扩散试验对疑似MRSA分离株的耐甲氧西林情况进行确认。135名新生儿中,28名(20.7%)鼻腔携带MRSA,30名(22.2%)脐部携带,10名(7.4%)鼻腔和脐部均携带MRSA。新生儿中未发现直肠VRE携带情况。因此,我们建议对入住新生儿重症监护病房的患者定期进行MRSA和VRE携带情况调查,这对于控制和预防医院感染极为有用。