Mulheron G W, Schomberg D W
Department of Obstetrics/Gynecology, Duke University Medical Center, Durham, NC 27710.
Endocrinology. 1990 Mar;126(3):1777-9. doi: 10.1210/endo-126-3-1777.
Freshly harvested granulosa cells (GC) from diethylstilbestrol (DES)-treated rats were examined for the presence of transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 2 mRNA by Northern analysis. TGF-beta 1 mRNA was not detectable, but hybridization of total RNA with a radiolabeled TGF-beta 2 cDNA probe revealed two mRNA species (5.1 and 3.6 kb) indicative of TGF-beta 2 mRNA. In response to FSH (50 ng/ml), relative TGF-beta 2 mRNA concentrations in cultured GC were 54% of control levels. Furthermore, the conditioned culture medium from FSH-treated GC contained significantly lower (p less than 0.01) TGF-beta-like activity relative to controls. These results demonstrate that rat GC express TGF-beta 2 mRNA which is regulatable by FSH in vitro.
通过Northern分析检测从己烯雌酚（DES）处理的大鼠新鲜收获的颗粒细胞（GC）中转化生长因子-β1（TGF-β1）和TGF-β2 mRNA的存在情况。未检测到TGF-β1 mRNA，但总RNA与放射性标记的TGF-β2 cDNA探针杂交显示出两种mRNA种类（5.1和3.6 kb），表明存在TGF-β2 mRNA。响应促卵泡激素（FSH，50 ng/ml），培养的GC中相对TGF-β2 mRNA浓度为对照水平的54%。此外，与对照相比，FSH处理的GC的条件培养基中TGF-β样活性显著降低（p<0.01）。这些结果表明大鼠GC表达TGF-β2 mRNA，其在体外可受FSH调节。
